DNA Purification

DNA purification is a crucial step in the process of preparation of samples. It removes enzymes and salts from lysed samples, or PCR products, prior to cloning and sequencing. It also eliminates unwanted PCR artifacts like nucleotides with no incorporation. DNA purification in molecular biological research is a critical step that requires careful planning for reliable, high-quality results.

The process of purifying DNA is accomplished by a variety of methods. The traditional DNA isolation methods contain http://www.mpsciences.com/2021/04/08/different-types-of-pcr-reagents/ a number of steps, including leukocyte separation or red blood cell lysis, to eliminate inhibitors of heme proteins of the PCR reaction. They also include deproteinization, RNAse treatment, ethanol and isopropanol precipitation, and then DNA elution. These protocols require specialized equipment, like an electrophoresis system and biosafety cabinets due the intercalating dyes used in electrophoresis gels.

Other DNA purification techniques use spin columns or 96-well filter plates that separate contamination by adsorbing them to the surface of the column or plate. These techniques can be very time-consuming, especially if you have many samples or if the columns need to be manually refilled.

Dipsticks dramatically reduce the number of steps involved in processing samples to three. They bind nucleic acid using a waxy substance made of cellulose and subsequently release them in the presence of water. This technique is particularly useful in low resource settings, such as remote sites and teaching labs. Its simplicity and speed (30 seconds for each sample) is a great fit for molecular diagnostics like the detection of disease, genotype screening and heterozygosity testing.